Journal: The Journal of Neuroscience

Article Title: Neural Stem/Progenitor Cells Participate in the Regenerative Response to Perinatal Hypoxia/Ischemia

doi: 10.1523/JNEUROSCI.1898-05.2006

Figure Lengend Snippet: Primer sets used in RT-PCR

Article Snippet: Only those genes that were induced or repressed at least twofold in each replication are listed in supplemental (available at www.jneurosci.org as supplemental material ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Transcript Primers EGFR (TaqMan) Applied Biosystems TaqMan Assay ID Rn00580398_m1 Notch1 (Lux) 5′-CAC GTA CTG CGA GCT GCC CTA CG[FAM]G-3′ 5′-GGC AGG TGC CTC CGT TCT-3′ 18S (TaqMan) Applied Biosystems TaqMan Assay ID Hs99999901_s1 18S (Lux) Invitrogen catalog #115HM-01 Open in a separate window Primer sets used in RT-PCR

Techniques: TaqMan Assay

Journal: The Journal of Neuroscience

Article Title: Neural Stem/Progenitor Cells Participate in the Regenerative Response to Perinatal Hypoxia/Ischemia

doi: 10.1523/JNEUROSCI.1898-05.2006

Figure Lengend Snippet: Signaling pathways involved in control of NSP fate are induced by perinatal H/I. Ipsilateral H/I and control hemispheres were dissected out after 48 h of recovery. A, Total RNA was isolated and amplified by qRT-PCR using primers specific for EGFR and Notch1 and normalized to expression of 18S. Values in parentheses indicate CV for the target gene in the sample groups; n = 10 for ipsilateral and contralateral conditions, and n = 4 for sham condition. *p < 0.05 versus sham; †p < 0.05 versus contralateral by pairwise fixed reallocation randomization. Immunostaining for Notch1 was performed on cryostat sections from H/I (B) and control (C) animals. In situ hybridization was performed on cryostat sections of contralateral (D, G), ipsilateral (E, H), and control (F, I) hemispheres using a digoxigenin-labeled RNA probe for Hes5 (D–F) and Hes1 (G–I). Arrows in E delineate the region of increased Hes5 expression. Inset in E shows high-magnification 100× Nomarski image of the Hes5+ region showing a subependymal Hes5+ cell body (arrowhead) with processes (arrow) projecting through the ependyma. There was no evident change in Hes1 expression. Scale bars: inset in E, 4 μm; F, 10 μm. cp, Choroid plexus; V, ventricle.

Article Snippet: Only those genes that were induced or repressed at least twofold in each replication are listed in supplemental (available at www.jneurosci.org as supplemental material ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Transcript Primers EGFR (TaqMan) Applied Biosystems TaqMan Assay ID Rn00580398_m1 Notch1 (Lux) 5′-CAC GTA CTG CGA GCT GCC CTA CG[FAM]G-3′ 5′-GGC AGG TGC CTC CGT TCT-3′ 18S (TaqMan) Applied Biosystems TaqMan Assay ID Hs99999901_s1 18S (Lux) Invitrogen catalog #115HM-01 Open in a separate window Primer sets used in RT-PCR

Techniques: Protein-Protein interactions, Control, Isolation, Amplification, Quantitative RT-PCR, Expressing, Immunostaining, In Situ Hybridization, Labeling

Journal: Inflammatory Intestinal Diseases

Article Title: Hypoxia Reduces the Transcription of Fibrotic Markers in the Intestinal Mucosa

doi: 10.1159/000513061

Figure Lengend Snippet: TaqMan gene expression assays

Article Snippet: Relative mRNA expression was determined by the comparative ∆∆Ct method using beta-actin (ACTB) as the reference gene. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Gene Full name TaqMan assay ID Human genes TGFβ Transforming growth factor beta Hs00998133_m1 ACTA2 Actin alpha 2, smooth muscle Hs00426835_g1 VIM Vimentin Hs00185584_m1 CDH1 Cadherin 1 Hs01023895_m1 CDH2 Cadherin 2 Hs00983056_m1 COL1A1 Collagen type I alpha 1 chain Hs00164004_m1 COL3A1 Collagen type III alpha 1 chain Hs00943809_m1 COL4A1 Collagen type IV alpha 1 chain Hs00266237_m1 MMP2 Matrix metallopeptidase 2 Hs01548727_m1 MMP3 Matrix metallopeptidase 3 Hs00968305_m1 MMP9 Matrix metallopeptidase 9 Hs00957562_m1 TIMP1 Tissue inhibitor of metalloproteinase 1 Hs01092512_g1 Mouse genes TGFβ Transforming growth factor beta Mm00524541_m1 ACTA2 Actin alpha 2, smooth muscle Mm00725412_s1 VIM Vimentin Mm01333430_m1 CDH1 Cadherin 1 Mm01247357_m1 CDH2 Cadherin 2 Mm01162497_m1 COL1A1 Collagen type I alpha 1 chain Mm00801666_g1 COL3A1 Collagen type III alpha 1 chain Mm00802300_m1 COL4A1 Collagen type IV alpha 1 chain Mm01210125_m1 MMP2 Matrix metallopeptidase 2 Mm00439498_m1 MMP3 Matrix metallopeptidase 3 Mm00440295_m1 MMP9 Matrix metallopeptidase 9 Mm00442991_m1 MMP13 Matrix metallopeptidase 13 Mm00439491_m1 TIMP1 Tissue inhibitor of metalloproteinase 1 Mm01341361_m1 Open in a separate window TaqMan gene expression assays Western Blot Total protein was harvested in M-PER lysis buffer (Thermo Fisher Scientific) supplemented with protease inhibitors (Roche Diagnostics, Mannheim, Germany).

Techniques: Gene Expression, TaqMan Assay

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Venn diagrams comparing the number of genes induced by PACAP, forskolin, dbcAMP, and/or NGF in PC12 cells after 6 h of treatment. A, diagram comparing the genes induced by PACAP (100 nM), forskolin (25 μM), and/or dbcAMP (1 mM). The experiments were conducted on an array of 15,000 genes, among which 27 seemed reproducibly activated by both PACAP, forskolin, and dbcAMP. B, diagram comparing the genes induced by PACAP (100 nM) and/or NGF (100 ng/ml). The experiments revealed that 20 genes were induced by both PACAP and NGF. C, diagram comparing the genes induced by forskolin (25 μM), dbcAMP (1 mM), and/or NGF (100 ng/ml). The experiments revealed only three genes commonly activated by cAMP stimulators and NGF. It should be noted that these genes were also induced by PACAP (see Tables 2–5).

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques:

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Genes induced by at least one factor, PACAP, forskolin, dbcAMP, and/or NGF Classification of the genes induced by a 6-h treatment with PACAP (100 nM), forskolin (25 μ M), dbcAMP (1 mM), and NGF (100 ng/ml). Transcripts were classified in decreasing order of magnitude of induction. Some transcripts with a ratio above 1.5 were not included in a particular category for a given treatment, if the data did not also satisfy microarray quality criteria (quality index >0.3). Bold characters indicate genes further investigated by real-time PCR as reported in .

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Ubiquitin Proteomics, Activation Assay, Sequencing, Virus

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Genes induced in presence of NGF Classification of the genes induced by a 6-h treatment with PACAP (100 nM), forskolin (25 μ M), dbcAMP (1 mM), or NGF (100 ng/ml). Transcripts were classified in decreasing order of magnitude of induction. Some transcripts with a ratio above 1.5 were not included in a particular category for a given treatment, if the data did not also satisfy microarray quality criteria (quality index >0.3). Bold characters indicate genes further investigated by real-time PCR as reported in .

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Derivative Assay, Ubiquitin Proteomics, Wilms Tumor Assay, Sequencing

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Genes induced only by PACAP Classification of the genes induced by a 6-h treatment with PACAP (100 nM), forskolin (25 μ M), dbcAMP (1 mM), or NGF (100 ng/ml). Transcripts were classified in decreasing order of magnitude of induction. Some transcripts with a ratio above 1.5 were not included in a particular category for a given treatment, if the data did not also satisfy microarray quality criteria (quality index >0.3). Bold characters indicate genes further investigated by real-time PCR as reported in .

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Sequencing, Ubiquitin Proteomics

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Genes induced by cAMP (forskolin and dbcAMP) Classification of the genes induced by a 6-h treatment with PACAP (100 nM), forskolin (250 nM), dbcAMP (10 mM), and/or NGF (100 ng/ml). Transcripts were classified in decreasing order of magnitude of induction. Some transcripts with a ratio above 1.5 were not included in a particular category for a given treatment, if the data did not also satisfy microarray quality criteria (quality index >0.3). Bold characters indicate genes further investigated by real-time PCR as reported in .

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Validation of microarray results by real-time PCR mRNA induction for 17 genes, found up-regulated by microarray, after a 6-h treatment with PACAP (100 nM), forskolin (25 μ M), dbcAMP (1 mM), or NGF (100 ng/ml). Genes were classified in ascending order of regulation by PACAP obtained by real-time PCR.

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques: Biomarker Discovery, Microarray, Real-time Polymerase Chain Reaction, Control

Journal: Molecular pharmacology

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells

doi: 10.1124/mol.107.044792

Figure Lengend Snippet: Time course of induction of PACAP target genes. Time course effect of PACAP (100 nM; red ), forskolin (25 μM; orange), dbcAMP (1 mM; blue), NGF (100 ng/ml; yellow), and medium (green) on the expression of various PACAP target genes. GATA binding protein 2 (Gata2), neuropilin 1 (Nrp1), adenylate cyclase activating polypeptide 1 receptor 1 (Pac-1), annexin A2 (Anx2), homer homolog 2 (Drosophila) (Homer 2), P450 (cytochrome) oxidoreductase (Por). Aldo-keto reductase family 1, member B8 (Akr1b8), villin 2 (Vil2), heat shock 22kDa protein 8 (Hspb8), early growth response 1 (Egr1), glutaredoxin (Glx), protein tyrosine phosphatase 4a1 (Ptp4a1). Growth arrest specific 1 (Gas1), antizyme inhibitor 1 (Azin1), ornithine decarboxylase, structural 1 (Odc), immediate early response 3 (Ier3) and regulator of G-protein signaling 2 (Rgs2). Each time point represents the mean -fold expression (± S.E.M.) compared with the time 0 h as measured by real-time PCR. Data were corrected using glyceraldehyde-3-phosphate dehydrogenase (Gapdh) signal as internal control.

Article Snippet: Bold characters indicate genes further investigated by real-time PCR as reported in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Gene Name Unigene Number GenBank ID PACAP Forskolin dbcAMP NGF Av S.E.M.

Techniques: Expressing, Binding Assay, Real-time Polymerase Chain Reaction, Control

Journal: Journal of materials science. Materials in medicine

Article Title: Microsphere-Based Scaffolds Encapsulating Tricalcium Phosphate And Hydroxyapatite For Bone Regeneration

doi: 10.1007/s10856-016-5734-1

Figure Lengend Snippet: Genes used for RT-qPCR analysis

Article Snippet: For quantification, the BLANK constructs at week 0 were designated as the calibrator group and GAPDH expression as the endogenous control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol TaqMan Assay ID Glyceraldehyde 3-phosphate dehydrogenase GAPDH Rn01775763_g1 Collagen type I COL1A1 Rn01463848_m1 Runt-related transcription factor 2 RUNX2 Rn01512298_m1 Bone gamma- carboxyglutamate protein BGLAP Rn00566386_g1 Integrin-binding sialoprotein IBSP Rn00561414_m1 Open in a separate window Genes used for RT-qPCR analysis 2.10 Histology and Immunohistochemistry (IHC) At 6 weeks, microsphere-based constructs (n = 3) were soaked in 30% w/v sucrose (Thermofisher Scientific, Waltham, MA) solution in PBS for 24 h. Afterward, the constructs were equilibrated in optimal cutting temperature embedding medium (OCT, Tissue-Tek, Torrance, CA) overnight at 37°C and then frozen at −20°C.

Techniques: TaqMan Assay

Journal:

Article Title: Mutations in the inosine monophosphate dehydrogenase 1 gene (IMPDH1) cause the RP10 form of autosomal dominant retinitis pigmentosa

doi:

Figure Lengend Snippet: Candidate genes in the RP10 region on 7q31.1

Article Snippet: Initial mapping data suggested that three mouse genes determined to have reduced expression levels in the crx − /crx − mice have human homologs located near the RP10 region on chromosome 7q ( ) ( 10 ). table ft1 table-wrap mode="anchored" t5 caption a7 Unigene number Gene name/description Human ortholog Expression levels Crx +/+ Crx −/− 27673 ESTs/osteoblast protein/novel glycosyltransferase NM_014888 4.2 2.0 38763 IMPDH1 NM_000883 13.7 2.0 27583 ESTs/same as predicted gene FLJ11350 NM_018396 2.1 0 Open in a separate window Candidate genes in the RP10 region on 7q31.1 Further analysis of these three genes determined that the genomic sequence corresponding to cDNA NM_014888 was located several megabases centromeric to the RP10 disease interval.

Techniques: Expressing

Journal: The Journal of Neuroscience

Article Title: Clonal Neural Stem Cells from Human Embryonic Stem Cell Colonies

doi: 10.1523/JNEUROSCI.3286-11.2012

Figure Lengend Snippet: TaqMan gene expression assays

Article Snippet: In cases where the highest expresser was not consistent among experiments the value for the highest expresser is <1 and is represented as a mean ± SEM. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Gene expression assay identification number 18S Hs99999901_s1 β -Actin Hs99999903_m1 OCT4 Hs01895601_u1 LIN28 Hs00702808_s1 SOX2 Hs00602736_s1 NES Hs00707120_s1 SOX1 Hs00534426_s1 PAX6 Hs00240871_m1 TUBB3 Hs00801390_s1 FOXA2 Hs00232764_m1 HNF4A Hs00766846_s1 GATA4 Hs00171403_m1 GATA1 Hs00231112_m1 T Hs00610080_m1 Open in a separate window TaqMan gene expression assays

Techniques: Gene Expression

Journal: Tissue Engineering. Part A

Article Title: In Vitro Osteogenic Potential of Human Mesenchymal Stem Cells Is Predicted by Runx2/Sox9 Ratio

doi: 10.1089/ten.tea.2014.0096

Figure Lengend Snippet: Primers/Probes Used for Real-Time Polymerase Chain Reaction

Article Snippet: Data analysis was performed using ddCT values, which were determined by normalization to 18S rRNA and samples harvested on day 0. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Forward primer Reverse primer Probe Applied biosystem reference number Runx2 AAG CAG TAT TTA CAA CAG AGG GTA CAA G GGT GCT CGG ATC CCA AAA CAT CAA ACA GCC TCT TCA GCA CAG TGA CAC Sox9 Hs00165814_m1 18S 431089E Open in a separate window Primers/Probes Used for Real-Time Polymerase Chain Reaction RNA interference The Neon Transfection System (Invitrogen) was used to transfect hMSCs (990V, 40 ms, 1 pulse) with 600 nM Sox9 (buffer concentration, OriGene).

Techniques:

Journal: Tissue Engineering. Part A

Article Title: In Vitro Osteogenic Potential of Human Mesenchymal Stem Cells Is Predicted by Runx2/Sox9 Ratio

doi: 10.1089/ten.tea.2014.0096

Figure Lengend Snippet: Sox9 gene expression of (A) on day 2 and 7 (n=12); (B) on day 14 and 21 (n=4), based on expression fold change to day 0 (mean±SEM *p<0.05); (C) Sox9 protein analysis: western blot and image analysis on day 7 either in control or dexamethasone (DEX) medium. Laminin B 1 served as internal control; one representative donor (n=3).

Article Snippet: Data analysis was performed using ddCT values, which were determined by normalization to 18S rRNA and samples harvested on day 0. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Forward primer Reverse primer Probe Applied biosystem reference number Runx2 AAG CAG TAT TTA CAA CAG AGG GTA CAA G GGT GCT CGG ATC CCA AAA CAT CAA ACA GCC TCT TCA GCA CAG TGA CAC Sox9 Hs00165814_m1 18S 431089E Open in a separate window Primers/Probes Used for Real-Time Polymerase Chain Reaction RNA interference The Neon Transfection System (Invitrogen) was used to transfect hMSCs (990V, 40 ms, 1 pulse) with 600 nM Sox9 (buffer concentration, OriGene).

Techniques: Gene Expression, Expressing, Western Blot, Control

Journal: Tissue Engineering. Part A

Article Title: In Vitro Osteogenic Potential of Human Mesenchymal Stem Cells Is Predicted by Runx2/Sox9 Ratio

doi: 10.1089/ten.tea.2014.0096

Figure Lengend Snippet: Runx2 gene expression (A) on day 2 and 7 (n=12); (B) on day 14 and 21 (n=4); Runx2/Sox9 ratio (C) on day 2 and 7 (n=12), (D) on day 14 and 21 (n=4), based on expression fold change to day 0 (mean±SEM *p<0.05).

Article Snippet: Data analysis was performed using ddCT values, which were determined by normalization to 18S rRNA and samples harvested on day 0. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Forward primer Reverse primer Probe Applied biosystem reference number Runx2 AAG CAG TAT TTA CAA CAG AGG GTA CAA G GGT GCT CGG ATC CCA AAA CAT CAA ACA GCC TCT TCA GCA CAG TGA CAC Sox9 Hs00165814_m1 18S 431089E Open in a separate window Primers/Probes Used for Real-Time Polymerase Chain Reaction RNA interference The Neon Transfection System (Invitrogen) was used to transfect hMSCs (990V, 40 ms, 1 pulse) with 600 nM Sox9 (buffer concentration, OriGene).

Techniques: Gene Expression, Expressing

Journal: Tissue Engineering. Part A

Article Title: In Vitro Osteogenic Potential of Human Mesenchymal Stem Cells Is Predicted by Runx2/Sox9 Ratio

doi: 10.1089/ten.tea.2014.0096

Figure Lengend Snippet: (A) Runx2/Sox9 ratio on day 7 (mean±SEM *p<0.05); (B) 45Ca incorporation on day 28 (mean±SEM *p<0.05), (C) alkaline phosphatase (ALP) activity on day 14, for 8 representative donors with low (n=4) and high (n=4) osteogenic potential (high osteogenic potential defined as above 100,000 CPMI/μg DNA on day 28; low osteogenic potential as below 80,000 CPMI/μg DNA on day 28); (D) ALP activity for donors with both low and high osteogenic potential (n=8); (E) correlation of 45Ca incorporation on day 28 with Runx2/Sox9 ratio on day 7; for unselected population of donors (n=12).

Article Snippet: Data analysis was performed using ddCT values, which were determined by normalization to 18S rRNA and samples harvested on day 0. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Forward primer Reverse primer Probe Applied biosystem reference number Runx2 AAG CAG TAT TTA CAA CAG AGG GTA CAA G GGT GCT CGG ATC CCA AAA CAT CAA ACA GCC TCT TCA GCA CAG TGA CAC Sox9 Hs00165814_m1 18S 431089E Open in a separate window Primers/Probes Used for Real-Time Polymerase Chain Reaction RNA interference The Neon Transfection System (Invitrogen) was used to transfect hMSCs (990V, 40 ms, 1 pulse) with 600 nM Sox9 (buffer concentration, OriGene).

Techniques: Activity Assay

Journal: Tissue Engineering. Part A

Article Title: In Vitro Osteogenic Potential of Human Mesenchymal Stem Cells Is Predicted by Runx2/Sox9 Ratio

doi: 10.1089/ten.tea.2014.0096

Figure Lengend Snippet: (A) Electroporation of hMSCs with Sox9 siRNA, gene expression after 48 h based on expression fold change to day 0; *indicates significant difference between Sox9 siRNA and controls (n=6, mean±SEM, *p<0.05), (B) protein analysis: western blot and image analysis of Sox9 protein after Sox9 siRNA electroporation (48 h) in control medium. Laminin B 1 served as internal control; one representative donor (n=3); (C) Runx2/Sox9 ratio after 48 h, one representative donor; (D) 45Ca incorporation on day 28 either in control or DEX medium; *indicates significant difference between Sox9 siRNA treatment and scrambled control and control, respectively (n=6, mean±SEM, *p<0.05); (E) staining of alizarin red S on day 28 after Sox9 siRNA treatment, either in control or DEX medium; representative images (scale bar: 200 μm); (F) optical density graphs in DEX medium; *indicates significant difference between Sox9 siRNA and control groups (n=6, mean±SEM *p<0.05). Color images available online at www.liebertpub.com/tea

Article Snippet: Data analysis was performed using ddCT values, which were determined by normalization to 18S rRNA and samples harvested on day 0. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Gene Forward primer Reverse primer Probe Applied biosystem reference number Runx2 AAG CAG TAT TTA CAA CAG AGG GTA CAA G GGT GCT CGG ATC CCA AAA CAT CAA ACA GCC TCT TCA GCA CAG TGA CAC Sox9 Hs00165814_m1 18S 431089E Open in a separate window Primers/Probes Used for Real-Time Polymerase Chain Reaction RNA interference The Neon Transfection System (Invitrogen) was used to transfect hMSCs (990V, 40 ms, 1 pulse) with 600 nM Sox9 (buffer concentration, OriGene).

Techniques: Electroporation, Gene Expression, Expressing, Western Blot, Control, Staining